Office Hours

  • Tuesday:

  • Wednesday:

  • Thurs:

  • or by appointment if available

 

Interests

 

Ruel McKnight

Associate Professor of

Chemistry & Biochemistry

ISC 327C
1 College Circle
Geneseo, NY 14454
585-245-5451
mcknight@geneseo.edu

Ruel M

Ruel McKnight has been a member of the Geneseo faculty since 2004.

Faculty Information

Research Interests

It is now well established that a wide range of important therapeutic drugs exert their effects by binding to DNA. These compounds generally interfere with the actions of many vital enzymes and protein factors involved DNA metabolism. However, a major limiting factor that exists with the use of these drugs is the occurrence of deleterious side effects. The McKnight research group is currently involved in the study of novel drug molecules binding to DNA using calorimetric, spectrophotometric, fluorometric and electrophoretic gel-based techniques. The long term goal is to develop a fundamental understanding of drug-DNA interactions and to use this knowledge as a guide to rationally design less toxic therapeutics.

Publications and Professional Activities

  • McKnight, R.E., Jackson, D.R. and Yokoyama, K., Temperature Dependence of Congo Red Binding to Amyloid Beta 12-28 , Eur. Biophys. J. 2013, 42 (6), 495-501.
  • McKnight, R.E., Insights into the Relative DNA Binding Affinity and Preferred Binding Mode of Homologous Compounds Using Isothermal Titration Calorimetry (ITC). (Book Chapter) In: Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry, Elkordy, A.A., Ed, InTech, 2013, ISBN 978-953-51-0947-1.
  • McKnight, R.E., Reisenauer, E., Pintado, M.V., Polasani, S.R. and Dixon, D.W., Substituent effect on the preferred DNA binding mode and affinity of a homologous series of naphthalene diimides, Bioorg. Med. Chem., Lett., 2011, 21 (14), 4288-4291.
  • Yokoyama, K., Fisher, A.D., Amori, A. R., Welchons, D., and McKnight, R.E., Spectroscopic and Calorimetric Studies of Congo Red Dye-Amyloid Beta Peptide Complexes, J. Biophys. Chem., 2010, 1 (3), 153-163.
  • McKnight, R.E., Onogul, B., Polasani, S.R., Gannon, M.K. II, and Detty, M.R., Substituent Control of DNA Binding Modes in a Series of Chalcogenoxanthylium Photosensitizers as Determined by Isothermal Titration Calorimetry and Topoisomerase I DNA Unwinding Assay, Bioorg. Med. Chem., 2008, 16 (24), 10221-10227.
  • McKnight, R.E., Ye, M., Ohulchanskyy, T., Sahabi, S., Wetzel, B.R., Wagner, S.J., Skripchenko, A. and Detty, M.R., Synthesis of Analogues of a Flexible Thiopyrylium Photosensitizer for Purging the Blood-Borne Pathogens and Binding Mode and Affinity Studies of their Complexes with DNA, Bioorg. Med. Chem., 2007, 15 (13), 4406-4418.
My Classes

Chem 216:
Organic Chemistry Laboratory

    Laboratory practices in representative organic preparations and procedures. Prerequisites: /Corequisite: CHEM 211 or CHEM 223. Offered every fall and spring

Chem 301:
Biochemistry Laboratory

    Students will be introduced to a selection of standard biochemical procedures such as DNA and protein purification, chromatographic separation, gel electrophoresis, dialysis, enzyme assay, and DNA analysis techniques. Prerequisites: CHEM 216 and CHEM 213 or CHEM 224. Co-requisites: CHEM 300 or CHEM 302.

Chem 304:
Biochemistry II

    Continuation of the chemistry of living organisms. The chemistry of nucleic acids is explored in depth and these principles are applied to understanding the tools that biochemists use in the laboratory. Regulation of genes and the molecular interactions of protein-DNA complexes are also investigated. The last part of the course focuses on the chemistry of metabolism and biosynthesis, along with the mechanisms of regulation of these processes. Prerequisites: CHEM 302. Offered every spring