Chem 301 - Biochemistry Laboratory SDS

Use the dropdown menu below to view safety data sheets (SDS) for each of the chemicals used in Chem 301, organized alphabetically by experiment.

Bradford Method & NADH Determination

• β-nicotinamide adenine dinucleotide reduceed disodium salt hydrate (NADH)
• Bovine serum albumin (BSA)
• Bradford reagent
• Lactate dehydrogenase (LDH)

DNA-Drug Binding Studies

• Agarose
• Daunomycin (daunorubicin)
• Distamycin (stallimycin) - ask your professor
• Ethidium bromide
• Loading buffer
  • Bromophenol Blue
  • Ficoll
  • Xylene Cyanol FF
• Plasmid DNA pUC19
• Sodium phosphate monobasic
• Tris-acetate-EDTA 50X (TAE)

Enzyme Kinetics & Tyrosinase Activity

• Levodopa (L-DOPA)
• Sodium phospate dibasic anhydrous
• Tyrosinase, from mushroom

Isolation of DNA from E. coli

• Bleach
• Chloroform
• Escherichia coli (E. coli)
• Ethanol
• Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA)
• Isoamyl alcohol (3-methyl-1-butanol)
• Lysozyme
• Sodium chloride
• Sodium dodecyl sulfate
• Sodium perchlorate
• Trisodium citrate dihydrate

LDH from Beef Heart

• β-nicotinamide adenine dinucleotide hydrate (NAD+)
• Ammonium sulfate
• Beef heart 
• Blue sepharose 6 Fast Flow
• Bovine serum albumin (BSA)
• Bradford reagent
• Lactic acid
• N-cylcohexyl-3-aminopropanesulfonic acid (CAPS)
• Potassium phosphate monobasic
• Sodium phosphate dibasic anhydrous

Pipetting

• Bovine serum albumin (BSA)
• Bradford reagent

SDS-PAGE of Proteins

• Alcohol dehydrogenase (ADH)
• Bio-Safe Coomassie G-250 stain
• Bovine serum albumin (BSA)
• Coomassie Brilliant Blue R-250 destaining solution
• Glyceraldehyde-3-phosphate dehydrogenase (G3PDH)
• Hemoglobin (Hb)
• Lactate dehydrogenase (LDH)
• Laemmli sample buffer, 2X or 4X
• Lysozyme
• Sodium phosphate dibasic anhydrous
• Tris-glycine-SDS (TGS) buffer
  • Glycine
  • Sodium dodecyl sulfate (SDS)
  • Tris (Trizma)